mouse miltenyi biotec (Miltenyi Biotec)
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Miltenyi Biotec
mouse miltenyi biotec
Mouse Miltenyi Biotec, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 656 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+miltenyi+biotec/CD117+MicroBeads%2C+mouse/pm42880243-678-224-225
Average 96 stars, based on 656 article reviews
Mouse Miltenyi Biotec, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 656 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+miltenyi+biotec/CD117+MicroBeads%2C+mouse/pm42880243-678-224-225
Average 96 stars, based on 656 article reviews
mouse miltenyi biotec - by Bioz Stars,
2026-11
96/100 stars
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Recombinant:Article Title: Protocol for isolation of live tumor-infiltrating immune cells from immunocompetent murine brains for high-dimensional profiling. Article Snippet: .. Antibodies DRAQ5TM (dilute 1:1000) BioLegend Cat#424101 DAPI (dilute 1:1000) ThermoFisher Scientific Cat#D3571 TruStain FcXTM PLUS (anti-mouse CD16/32) (dilute 1:100) BioLegend Cat#156603 BV605 rat anti-CD11b clone M1/70 (RUO) (dilute 1:100) BD HorizonTM Cat#563015; RRID:AB_2737951 Chemicals, peptides, and recombinant proteins Narketan 10 100 mg/ml solution for injection (ketamine) Vetoquinol B.V. QN01AX03 Sedastart 1 mg/ml solution for injection (medetomidine) Ast Farma B.V. QN05CM91 K2-EDTA Sigma-Aldrich Cat#03660 Critical commercial assays Tumor Dissociation Kit, Article Title: Replaying germinal center evolution on a quantified affinity landscape. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-CD16/32 (Fc block) (clone 2.4G2) BD Biosciences RRID: AB_394656 Anti-B220 BV785 (clone RA3-6B2) Biolegend RRID: AB_11218795 Anti-CD38 APC (clone 90) Invitrogen RRID: AB_469382 Anti-CD95 (Fas) Pe-Cy7 (clone Jo2) BD Biosciences RRID: AB_396768 Streptavidin BV421 Biolegend Cat# 405226 Anti-Igκ PE (clone 187.1) BD Biosciences Cat# 559940; RRID:AB_397384 Streptavidin PE Thermo Fisher Scientific Cat# S866 Anti-TCRβ APC-eFluor780 (clone H57–597) eBioscience RRID: AB_1272173 Anti-c-Myc FITC (CYMC-45F) Immunology Consultants Lab Cat# CYMC-45F Anti-CD35 Cy3 (clone 8C12) In house N/A Anti-GFP BioLegend Cat# 902605; RRID: AB_2734671 TotalSeqTM-C0307 anti-mouse Hashtag Antibody (1–16) BioLegend Various Bacterial and virus strains E. Coli New England Biolabs Cat# C3020K Biological samples Plasmodium Chabaudi BEI Resources Cat# MRA-741 Chemicals, peptides, and recombinant proteins Imject Alum Thermo Fisher Scientific Cat# 77161 Alhydrogel adjuvant 2% InvivoGen Cat# vac-alu-250 Normal Chicken IgY (egg-derived) Exalpha Biologicals Cat# IgY-100 ACK lysing buffer Thermo Fisher Scientific Cat# A10492-01 Tween20 Sigma-Aldrich Cat# P9416-50ML Bovine Serum Albumin (BSA) Sigma-Aldrich Cat# A9647-500G Fetal bovine serum (FBS) Thermo Fisher Scientific Cat# A3160401 Buffer TCL Qiagen Cat# 1031576 EDTA Spectrum Laboratories Cat# 40520000-3 Trizol LS Thermo Fisher Scientific Cat# 10296010 Beta mercaptoethanol Thermo Fisher Scientific Cat #21985023 ECL substrate Pierce N/A Critical commercial assays Zeba desalting column purification Thermo Fisher Scientific Cat# 89883 Protein-G Sepharose Fisher Scientific Cat# 45-000-116 Ni-INDIGO Agarose Beads PureCube Cat# 75105 MACS Cell Separation Column LS Miltenyi Biotec Cat# 130-042-401 CD43 (Ly-48) microbeads, Article Title: Protocol for minimized-bias profiling of liver and visceral adipose tissue in mice using integrated single-nucleus RNA sequencing. Article Snippet: .. Biological samples Male wild-type C57BL/6J mice, 8 weeks old at diet initiation; tissues collected after 26 weeks of western diet feeding Janvier Labs Ethics approval #47097-2024012516265646 v4 Chemicals, peptides, and recombinant proteins PBS Gibco Cat#10010023 DMEM Gibco Cat#10566016 Absolute ethanol Major supplier N/A Nuclease-free water Major supplier N/A DAPI Staining Solution MILTENYI BIOTEC Cat#130-111-570 RNase inhibitor, 40000 U/mL New England Biolabs Cat#M0314 BD Stain Buffer (FBS) BD Biosciences Cat#554656 Proteinase K, 800 U/mL New England Biolabs Cat#P8107S Vybrant® DyeCycleTM Green Stain Thermo Fisher Cat#V35004 Critical commercial assays Nuclei Extraction Buffer MILTENYI BIOTEC Cat#130-128-024 Liver Dissociation Kit, Injection:Article Title: Protocol for isolation of live tumor-infiltrating immune cells from immunocompetent murine brains for high-dimensional profiling. Article Snippet: .. Antibodies DRAQ5TM (dilute 1:1000) BioLegend Cat#424101 DAPI (dilute 1:1000) ThermoFisher Scientific Cat#D3571 TruStain FcXTM PLUS (anti-mouse CD16/32) (dilute 1:100) BioLegend Cat#156603 BV605 rat anti-CD11b clone M1/70 (RUO) (dilute 1:100) BD HorizonTM Cat#563015; RRID:AB_2737951 Chemicals, peptides, and recombinant proteins Narketan 10 100 mg/ml solution for injection (ketamine) Vetoquinol B.V. QN01AX03 Sedastart 1 mg/ml solution for injection (medetomidine) Ast Farma B.V. QN05CM91 K2-EDTA Sigma-Aldrich Cat#03660 Critical commercial assays Tumor Dissociation Kit, Magnetic Cell Separation:Article Title: Protocol for isolation of live tumor-infiltrating immune cells from immunocompetent murine brains for high-dimensional profiling. Article Snippet: .. Antibodies DRAQ5TM (dilute 1:1000) BioLegend Cat#424101 DAPI (dilute 1:1000) ThermoFisher Scientific Cat#D3571 TruStain FcXTM PLUS (anti-mouse CD16/32) (dilute 1:100) BioLegend Cat#156603 BV605 rat anti-CD11b clone M1/70 (RUO) (dilute 1:100) BD HorizonTM Cat#563015; RRID:AB_2737951 Chemicals, peptides, and recombinant proteins Narketan 10 100 mg/ml solution for injection (ketamine) Vetoquinol B.V. QN01AX03 Sedastart 1 mg/ml solution for injection (medetomidine) Ast Farma B.V. QN05CM91 K2-EDTA Sigma-Aldrich Cat#03660 Critical commercial assays Tumor Dissociation Kit, Article Title: Replaying germinal center evolution on a quantified affinity landscape. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-CD16/32 (Fc block) (clone 2.4G2) BD Biosciences RRID: AB_394656 Anti-B220 BV785 (clone RA3-6B2) Biolegend RRID: AB_11218795 Anti-CD38 APC (clone 90) Invitrogen RRID: AB_469382 Anti-CD95 (Fas) Pe-Cy7 (clone Jo2) BD Biosciences RRID: AB_396768 Streptavidin BV421 Biolegend Cat# 405226 Anti-Igκ PE (clone 187.1) BD Biosciences Cat# 559940; RRID:AB_397384 Streptavidin PE Thermo Fisher Scientific Cat# S866 Anti-TCRβ APC-eFluor780 (clone H57–597) eBioscience RRID: AB_1272173 Anti-c-Myc FITC (CYMC-45F) Immunology Consultants Lab Cat# CYMC-45F Anti-CD35 Cy3 (clone 8C12) In house N/A Anti-GFP BioLegend Cat# 902605; RRID: AB_2734671 TotalSeqTM-C0307 anti-mouse Hashtag Antibody (1–16) BioLegend Various Bacterial and virus strains E. Coli New England Biolabs Cat# C3020K Biological samples Plasmodium Chabaudi BEI Resources Cat# MRA-741 Chemicals, peptides, and recombinant proteins Imject Alum Thermo Fisher Scientific Cat# 77161 Alhydrogel adjuvant 2% InvivoGen Cat# vac-alu-250 Normal Chicken IgY (egg-derived) Exalpha Biologicals Cat# IgY-100 ACK lysing buffer Thermo Fisher Scientific Cat# A10492-01 Tween20 Sigma-Aldrich Cat# P9416-50ML Bovine Serum Albumin (BSA) Sigma-Aldrich Cat# A9647-500G Fetal bovine serum (FBS) Thermo Fisher Scientific Cat# A3160401 Buffer TCL Qiagen Cat# 1031576 EDTA Spectrum Laboratories Cat# 40520000-3 Trizol LS Thermo Fisher Scientific Cat# 10296010 Beta mercaptoethanol Thermo Fisher Scientific Cat #21985023 ECL substrate Pierce N/A Critical commercial assays Zeba desalting column purification Thermo Fisher Scientific Cat# 89883 Protein-G Sepharose Fisher Scientific Cat# 45-000-116 Ni-INDIGO Agarose Beads PureCube Cat# 75105 MACS Cell Separation Column LS Miltenyi Biotec Cat# 130-042-401 CD43 (Ly-48) microbeads, Article Title: Protocol for minimized-bias profiling of liver and visceral adipose tissue in mice using integrated single-nucleus RNA sequencing. Article Snippet: .. Biological samples Male wild-type C57BL/6J mice, 8 weeks old at diet initiation; tissues collected after 26 weeks of western diet feeding Janvier Labs Ethics approval #47097-2024012516265646 v4 Chemicals, peptides, and recombinant proteins PBS Gibco Cat#10010023 DMEM Gibco Cat#10566016 Absolute ethanol Major supplier N/A Nuclease-free water Major supplier N/A DAPI Staining Solution MILTENYI BIOTEC Cat#130-111-570 RNase inhibitor, 40000 U/mL New England Biolabs Cat#M0314 BD Stain Buffer (FBS) BD Biosciences Cat#554656 Proteinase K, 800 U/mL New England Biolabs Cat#P8107S Vybrant® DyeCycleTM Green Stain Thermo Fisher Cat#V35004 Critical commercial assays Nuclei Extraction Buffer MILTENYI BIOTEC Cat#130-128-024 Liver Dissociation Kit, Staining:Article Title: Protocol for isolation of live tumor-infiltrating immune cells from immunocompetent murine brains for high-dimensional profiling. Article Snippet: .. Antibodies DRAQ5TM (dilute 1:1000) BioLegend Cat#424101 DAPI (dilute 1:1000) ThermoFisher Scientific Cat#D3571 TruStain FcXTM PLUS (anti-mouse CD16/32) (dilute 1:100) BioLegend Cat#156603 BV605 rat anti-CD11b clone M1/70 (RUO) (dilute 1:100) BD HorizonTM Cat#563015; RRID:AB_2737951 Chemicals, peptides, and recombinant proteins Narketan 10 100 mg/ml solution for injection (ketamine) Vetoquinol B.V. QN01AX03 Sedastart 1 mg/ml solution for injection (medetomidine) Ast Farma B.V. QN05CM91 K2-EDTA Sigma-Aldrich Cat#03660 Critical commercial assays Tumor Dissociation Kit, Article Title: Gemella morbillorum promotes colorectal tumorigenesis through barrier disruption and invasion-dependent DNA damage. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER PitStop2 MedChemExpress HY-115604 Triton X-100 Sigma-Aldrich T8787 Horse Serum Sigma-Aldrich H1270 Streptomycin sulfate salt Sigma-Aldrich S1277 Brain Heart Infusion (BHI) Sigma-Aldrich 53286 Trypticase Soy Broth Merck Millipore 22092 Yeast Extract Carl Roth 2363.2 Potassium dihydrogen phosphate (KH2PO4) Sigma-Aldrich P749.1 Glucose Merck Millipore 1.08337 Sodium carbonate (NA2CO3) VWR 27771 Mucin from procine stomach Sigma-Aldrich M2378 Cysteine-HCl Sigma-Aldrich C7477 Menadione Merck Millipore M5625 Hemin Merck Millipore 51280 Lipofectamine RNAiMAX ThermoFischer Scientific 13778075 Silencer Select Negative Control No. 1 siRNA ThermoFischer Scientific 4390843 eBioscience Cell Proliferation Dye eFluor 450 ThermoFischer Scientific 65-0842-85 Fast Green FCF dye Sigma-Aldrich F7252 TrypLE Express Enzyme (1X), no phenol red Gibco 12604021 Y-27632 (ROCK inhibitor) Tocris/Bio-Techne 1254 Matrigel Corning 356231 Azoxymethane Sigma-Aldrich A5486 Anaerobic Basal Broth Neolab M1636 Agar ThermoFischer Scientific 10572775 Horse Blood (Defibrinated, Sterile) TEBU BIO 007CL1500-50D Fluorescein isothiocyanate–dextran Sigma-Aldrich 46944 DTT Carl Roth 6908.2 Phorbol 12-myristate 13-acetate (PMA) Sigma-Aldrich P1585 Ionomycin ThermoFischer Scientific J62448MCR RPMI 1640 Medium, GlutaMAX Supplement Gibco 61870010 Erylysis-Buffer Morphisto 12972.00 Fluoromount-G Mounting Medium Invitrogen 00-4958-02 Brefeldin A Solution (1000X) Invitrogen 00-4506-51 Phenex-PTFE Syringe Filters Phenomenex AF0-3202-52 Advanced DMEM/F-12 ThermoFischer Scientific 12634028 B-27 Supplement (S50X), minus vitamin A ThermoFischer Scientific 12587010 GlutaMAX Supplement Gibco 35050061 HEPES (1M) ThermoFischer Scientific 15630056 N-Acetyl-L-cysteine Sigma-Aldrich A9165 Nicotinamide Sigma-Aldrich N0636 Noggin conditioned medium ImmunoPrecise N002 R-spondin I conditioned medium ImmunoPrecise R001 Human EGF Peprotech AF-100-15 A83-01 Tocris 2939 Prostaglandin E2 Tocris 2296 SB202190 Sigma-Aldrich S7076 Wnt surrogate U-Protein Express N001 Primocin InvivoGen ant-pm-05 Aqueous Glutaraldehyde EM Grade 25% Electron Microscopy Sciences 16220 Trihydrate Sodium Cacodylate Electron Microscopy Sciences 12310 (Continued on next page) ll OPEN ACCESS Article e3 Cell Host & Microbe 34, 1–17.e1–e12, November 11, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Osmium Tetroxide Aqueous Solution Electron Microscopy Sciences 19170 AGAR 100 Resin Agar Scientific R1031 Opti-MEM I Reduced Serum Medium ThermoFischer Scientific 31985062 Trypsin-EDTA (0.05%), phenol red ThermoFischer Scientific 25300062 Fetal Bovine Serum, Value ThermoFischer Scientific A5256701 GelGreen VWR 730–1535 Sodium chloride (NaCl) MP Biomedicals 194738 EDTA ThermoFischer Scientific 15575020 Tris Carl Roth 4855.3 Hydrochloric acid (HCl) Carl Roth 2607.1 DMSO ITW Reagents A3672,0050 Sodium hydroxide (NaOH) Carl Roth 6785.1 Eosin Y solution Carl Roth 229C.1 ROTIHistofix Carl Roth P087.5 Hematoxylin Solution, Gill No. 3 Sigma-Aldrich GHS316 Stealth siRNA ANXA2 ThermoFischer Scientific HSS179173 CAT#1299001 Critical commercial assays Opal 520 Reagent Pack Akoya Biosciences FP1487001KT Opal 480 Reagent Pack Akoya Biosciences FP1500001KT eBioscience Foxp3 / Transcription Factor Staining Buffer Set ThermoFischer Scientific 00-5523-00 LIVE/DEAD Fixable Near-IR Dead Cell Stain Kit Invitrogen L34975 CellTrace CFSE Cell Proliferation Kit, for flow cytometry ThermoFischer Scientific C34554 SignalStain DAB Substrate Kit CST 8059 Mouse IL-22 DuoSet ELISA R&D Systems DY582 Tumor Dissociation Kit, human Miltenyi Biotec 130-095-929 Lamina Propria Dissociation Kit, Article Title: Protocol for minimized-bias profiling of liver and visceral adipose tissue in mice using integrated single-nucleus RNA sequencing. Article Snippet: .. Biological samples Male wild-type C57BL/6J mice, 8 weeks old at diet initiation; tissues collected after 26 weeks of western diet feeding Janvier Labs Ethics approval #47097-2024012516265646 v4 Chemicals, peptides, and recombinant proteins PBS Gibco Cat#10010023 DMEM Gibco Cat#10566016 Absolute ethanol Major supplier N/A Nuclease-free water Major supplier N/A DAPI Staining Solution MILTENYI BIOTEC Cat#130-111-570 RNase inhibitor, 40000 U/mL New England Biolabs Cat#M0314 BD Stain Buffer (FBS) BD Biosciences Cat#554656 Proteinase K, 800 U/mL New England Biolabs Cat#P8107S Vybrant® DyeCycleTM Green Stain Thermo Fisher Cat#V35004 Critical commercial assays Nuclei Extraction Buffer MILTENYI BIOTEC Cat#130-128-024 Liver Dissociation Kit, Electron Microscopy:Article Title: Gemella morbillorum promotes colorectal tumorigenesis through barrier disruption and invasion-dependent DNA damage. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER PitStop2 MedChemExpress HY-115604 Triton X-100 Sigma-Aldrich T8787 Horse Serum Sigma-Aldrich H1270 Streptomycin sulfate salt Sigma-Aldrich S1277 Brain Heart Infusion (BHI) Sigma-Aldrich 53286 Trypticase Soy Broth Merck Millipore 22092 Yeast Extract Carl Roth 2363.2 Potassium dihydrogen phosphate (KH2PO4) Sigma-Aldrich P749.1 Glucose Merck Millipore 1.08337 Sodium carbonate (NA2CO3) VWR 27771 Mucin from procine stomach Sigma-Aldrich M2378 Cysteine-HCl Sigma-Aldrich C7477 Menadione Merck Millipore M5625 Hemin Merck Millipore 51280 Lipofectamine RNAiMAX ThermoFischer Scientific 13778075 Silencer Select Negative Control No. 1 siRNA ThermoFischer Scientific 4390843 eBioscience Cell Proliferation Dye eFluor 450 ThermoFischer Scientific 65-0842-85 Fast Green FCF dye Sigma-Aldrich F7252 TrypLE Express Enzyme (1X), no phenol red Gibco 12604021 Y-27632 (ROCK inhibitor) Tocris/Bio-Techne 1254 Matrigel Corning 356231 Azoxymethane Sigma-Aldrich A5486 Anaerobic Basal Broth Neolab M1636 Agar ThermoFischer Scientific 10572775 Horse Blood (Defibrinated, Sterile) TEBU BIO 007CL1500-50D Fluorescein isothiocyanate–dextran Sigma-Aldrich 46944 DTT Carl Roth 6908.2 Phorbol 12-myristate 13-acetate (PMA) Sigma-Aldrich P1585 Ionomycin ThermoFischer Scientific J62448MCR RPMI 1640 Medium, GlutaMAX Supplement Gibco 61870010 Erylysis-Buffer Morphisto 12972.00 Fluoromount-G Mounting Medium Invitrogen 00-4958-02 Brefeldin A Solution (1000X) Invitrogen 00-4506-51 Phenex-PTFE Syringe Filters Phenomenex AF0-3202-52 Advanced DMEM/F-12 ThermoFischer Scientific 12634028 B-27 Supplement (S50X), minus vitamin A ThermoFischer Scientific 12587010 GlutaMAX Supplement Gibco 35050061 HEPES (1M) ThermoFischer Scientific 15630056 N-Acetyl-L-cysteine Sigma-Aldrich A9165 Nicotinamide Sigma-Aldrich N0636 Noggin conditioned medium ImmunoPrecise N002 R-spondin I conditioned medium ImmunoPrecise R001 Human EGF Peprotech AF-100-15 A83-01 Tocris 2939 Prostaglandin E2 Tocris 2296 SB202190 Sigma-Aldrich S7076 Wnt surrogate U-Protein Express N001 Primocin InvivoGen ant-pm-05 Aqueous Glutaraldehyde EM Grade 25% Electron Microscopy Sciences 16220 Trihydrate Sodium Cacodylate Electron Microscopy Sciences 12310 (Continued on next page) ll OPEN ACCESS Article e3 Cell Host & Microbe 34, 1–17.e1–e12, November 11, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Osmium Tetroxide Aqueous Solution Electron Microscopy Sciences 19170 AGAR 100 Resin Agar Scientific R1031 Opti-MEM I Reduced Serum Medium ThermoFischer Scientific 31985062 Trypsin-EDTA (0.05%), phenol red ThermoFischer Scientific 25300062 Fetal Bovine Serum, Value ThermoFischer Scientific A5256701 GelGreen VWR 730–1535 Sodium chloride (NaCl) MP Biomedicals 194738 EDTA ThermoFischer Scientific 15575020 Tris Carl Roth 4855.3 Hydrochloric acid (HCl) Carl Roth 2607.1 DMSO ITW Reagents A3672,0050 Sodium hydroxide (NaOH) Carl Roth 6785.1 Eosin Y solution Carl Roth 229C.1 ROTIHistofix Carl Roth P087.5 Hematoxylin Solution, Gill No. 3 Sigma-Aldrich GHS316 Stealth siRNA ANXA2 ThermoFischer Scientific HSS179173 CAT#1299001 Critical commercial assays Opal 520 Reagent Pack Akoya Biosciences FP1487001KT Opal 480 Reagent Pack Akoya Biosciences FP1500001KT eBioscience Foxp3 / Transcription Factor Staining Buffer Set ThermoFischer Scientific 00-5523-00 LIVE/DEAD Fixable Near-IR Dead Cell Stain Kit Invitrogen L34975 CellTrace CFSE Cell Proliferation Kit, for flow cytometry ThermoFischer Scientific C34554 SignalStain DAB Substrate Kit CST 8059 Mouse IL-22 DuoSet ELISA R&D Systems DY582 Tumor Dissociation Kit, human Miltenyi Biotec 130-095-929 Lamina Propria Dissociation Kit, Chloramphenicol Acetyltransferase Assay:Article Title: Gemella morbillorum promotes colorectal tumorigenesis through barrier disruption and invasion-dependent DNA damage. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER PitStop2 MedChemExpress HY-115604 Triton X-100 Sigma-Aldrich T8787 Horse Serum Sigma-Aldrich H1270 Streptomycin sulfate salt Sigma-Aldrich S1277 Brain Heart Infusion (BHI) Sigma-Aldrich 53286 Trypticase Soy Broth Merck Millipore 22092 Yeast Extract Carl Roth 2363.2 Potassium dihydrogen phosphate (KH2PO4) Sigma-Aldrich P749.1 Glucose Merck Millipore 1.08337 Sodium carbonate (NA2CO3) VWR 27771 Mucin from procine stomach Sigma-Aldrich M2378 Cysteine-HCl Sigma-Aldrich C7477 Menadione Merck Millipore M5625 Hemin Merck Millipore 51280 Lipofectamine RNAiMAX ThermoFischer Scientific 13778075 Silencer Select Negative Control No. 1 siRNA ThermoFischer Scientific 4390843 eBioscience Cell Proliferation Dye eFluor 450 ThermoFischer Scientific 65-0842-85 Fast Green FCF dye Sigma-Aldrich F7252 TrypLE Express Enzyme (1X), no phenol red Gibco 12604021 Y-27632 (ROCK inhibitor) Tocris/Bio-Techne 1254 Matrigel Corning 356231 Azoxymethane Sigma-Aldrich A5486 Anaerobic Basal Broth Neolab M1636 Agar ThermoFischer Scientific 10572775 Horse Blood (Defibrinated, Sterile) TEBU BIO 007CL1500-50D Fluorescein isothiocyanate–dextran Sigma-Aldrich 46944 DTT Carl Roth 6908.2 Phorbol 12-myristate 13-acetate (PMA) Sigma-Aldrich P1585 Ionomycin ThermoFischer Scientific J62448MCR RPMI 1640 Medium, GlutaMAX Supplement Gibco 61870010 Erylysis-Buffer Morphisto 12972.00 Fluoromount-G Mounting Medium Invitrogen 00-4958-02 Brefeldin A Solution (1000X) Invitrogen 00-4506-51 Phenex-PTFE Syringe Filters Phenomenex AF0-3202-52 Advanced DMEM/F-12 ThermoFischer Scientific 12634028 B-27 Supplement (S50X), minus vitamin A ThermoFischer Scientific 12587010 GlutaMAX Supplement Gibco 35050061 HEPES (1M) ThermoFischer Scientific 15630056 N-Acetyl-L-cysteine Sigma-Aldrich A9165 Nicotinamide Sigma-Aldrich N0636 Noggin conditioned medium ImmunoPrecise N002 R-spondin I conditioned medium ImmunoPrecise R001 Human EGF Peprotech AF-100-15 A83-01 Tocris 2939 Prostaglandin E2 Tocris 2296 SB202190 Sigma-Aldrich S7076 Wnt surrogate U-Protein Express N001 Primocin InvivoGen ant-pm-05 Aqueous Glutaraldehyde EM Grade 25% Electron Microscopy Sciences 16220 Trihydrate Sodium Cacodylate Electron Microscopy Sciences 12310 (Continued on next page) ll OPEN ACCESS Article e3 Cell Host & Microbe 34, 1–17.e1–e12, November 11, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Osmium Tetroxide Aqueous Solution Electron Microscopy Sciences 19170 AGAR 100 Resin Agar Scientific R1031 Opti-MEM I Reduced Serum Medium ThermoFischer Scientific 31985062 Trypsin-EDTA (0.05%), phenol red ThermoFischer Scientific 25300062 Fetal Bovine Serum, Value ThermoFischer Scientific A5256701 GelGreen VWR 730–1535 Sodium chloride (NaCl) MP Biomedicals 194738 EDTA ThermoFischer Scientific 15575020 Tris Carl Roth 4855.3 Hydrochloric acid (HCl) Carl Roth 2607.1 DMSO ITW Reagents A3672,0050 Sodium hydroxide (NaOH) Carl Roth 6785.1 Eosin Y solution Carl Roth 229C.1 ROTIHistofix Carl Roth P087.5 Hematoxylin Solution, Gill No. 3 Sigma-Aldrich GHS316 Stealth siRNA ANXA2 ThermoFischer Scientific HSS179173 CAT#1299001 Critical commercial assays Opal 520 Reagent Pack Akoya Biosciences FP1487001KT Opal 480 Reagent Pack Akoya Biosciences FP1500001KT eBioscience Foxp3 / Transcription Factor Staining Buffer Set ThermoFischer Scientific 00-5523-00 LIVE/DEAD Fixable Near-IR Dead Cell Stain Kit Invitrogen L34975 CellTrace CFSE Cell Proliferation Kit, for flow cytometry ThermoFischer Scientific C34554 SignalStain DAB Substrate Kit CST 8059 Mouse IL-22 DuoSet ELISA R&D Systems DY582 Tumor Dissociation Kit, human Miltenyi Biotec 130-095-929 Lamina Propria Dissociation Kit, Flow Cytometry:Article Title: Gemella morbillorum promotes colorectal tumorigenesis through barrier disruption and invasion-dependent DNA damage. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER PitStop2 MedChemExpress HY-115604 Triton X-100 Sigma-Aldrich T8787 Horse Serum Sigma-Aldrich H1270 Streptomycin sulfate salt Sigma-Aldrich S1277 Brain Heart Infusion (BHI) Sigma-Aldrich 53286 Trypticase Soy Broth Merck Millipore 22092 Yeast Extract Carl Roth 2363.2 Potassium dihydrogen phosphate (KH2PO4) Sigma-Aldrich P749.1 Glucose Merck Millipore 1.08337 Sodium carbonate (NA2CO3) VWR 27771 Mucin from procine stomach Sigma-Aldrich M2378 Cysteine-HCl Sigma-Aldrich C7477 Menadione Merck Millipore M5625 Hemin Merck Millipore 51280 Lipofectamine RNAiMAX ThermoFischer Scientific 13778075 Silencer Select Negative Control No. 1 siRNA ThermoFischer Scientific 4390843 eBioscience Cell Proliferation Dye eFluor 450 ThermoFischer Scientific 65-0842-85 Fast Green FCF dye Sigma-Aldrich F7252 TrypLE Express Enzyme (1X), no phenol red Gibco 12604021 Y-27632 (ROCK inhibitor) Tocris/Bio-Techne 1254 Matrigel Corning 356231 Azoxymethane Sigma-Aldrich A5486 Anaerobic Basal Broth Neolab M1636 Agar ThermoFischer Scientific 10572775 Horse Blood (Defibrinated, Sterile) TEBU BIO 007CL1500-50D Fluorescein isothiocyanate–dextran Sigma-Aldrich 46944 DTT Carl Roth 6908.2 Phorbol 12-myristate 13-acetate (PMA) Sigma-Aldrich P1585 Ionomycin ThermoFischer Scientific J62448MCR RPMI 1640 Medium, GlutaMAX Supplement Gibco 61870010 Erylysis-Buffer Morphisto 12972.00 Fluoromount-G Mounting Medium Invitrogen 00-4958-02 Brefeldin A Solution (1000X) Invitrogen 00-4506-51 Phenex-PTFE Syringe Filters Phenomenex AF0-3202-52 Advanced DMEM/F-12 ThermoFischer Scientific 12634028 B-27 Supplement (S50X), minus vitamin A ThermoFischer Scientific 12587010 GlutaMAX Supplement Gibco 35050061 HEPES (1M) ThermoFischer Scientific 15630056 N-Acetyl-L-cysteine Sigma-Aldrich A9165 Nicotinamide Sigma-Aldrich N0636 Noggin conditioned medium ImmunoPrecise N002 R-spondin I conditioned medium ImmunoPrecise R001 Human EGF Peprotech AF-100-15 A83-01 Tocris 2939 Prostaglandin E2 Tocris 2296 SB202190 Sigma-Aldrich S7076 Wnt surrogate U-Protein Express N001 Primocin InvivoGen ant-pm-05 Aqueous Glutaraldehyde EM Grade 25% Electron Microscopy Sciences 16220 Trihydrate Sodium Cacodylate Electron Microscopy Sciences 12310 (Continued on next page) ll OPEN ACCESS Article e3 Cell Host & Microbe 34, 1–17.e1–e12, November 11, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Osmium Tetroxide Aqueous Solution Electron Microscopy Sciences 19170 AGAR 100 Resin Agar Scientific R1031 Opti-MEM I Reduced Serum Medium ThermoFischer Scientific 31985062 Trypsin-EDTA (0.05%), phenol red ThermoFischer Scientific 25300062 Fetal Bovine Serum, Value ThermoFischer Scientific A5256701 GelGreen VWR 730–1535 Sodium chloride (NaCl) MP Biomedicals 194738 EDTA ThermoFischer Scientific 15575020 Tris Carl Roth 4855.3 Hydrochloric acid (HCl) Carl Roth 2607.1 DMSO ITW Reagents A3672,0050 Sodium hydroxide (NaOH) Carl Roth 6785.1 Eosin Y solution Carl Roth 229C.1 ROTIHistofix Carl Roth P087.5 Hematoxylin Solution, Gill No. 3 Sigma-Aldrich GHS316 Stealth siRNA ANXA2 ThermoFischer Scientific HSS179173 CAT#1299001 Critical commercial assays Opal 520 Reagent Pack Akoya Biosciences FP1487001KT Opal 480 Reagent Pack Akoya Biosciences FP1500001KT eBioscience Foxp3 / Transcription Factor Staining Buffer Set ThermoFischer Scientific 00-5523-00 LIVE/DEAD Fixable Near-IR Dead Cell Stain Kit Invitrogen L34975 CellTrace CFSE Cell Proliferation Kit, for flow cytometry ThermoFischer Scientific C34554 SignalStain DAB Substrate Kit CST 8059 Mouse IL-22 DuoSet ELISA R&D Systems DY582 Tumor Dissociation Kit, human Miltenyi Biotec 130-095-929 Lamina Propria Dissociation Kit, Enzyme-linked Immunosorbent Assay:Article Title: Gemella morbillorum promotes colorectal tumorigenesis through barrier disruption and invasion-dependent DNA damage. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER PitStop2 MedChemExpress HY-115604 Triton X-100 Sigma-Aldrich T8787 Horse Serum Sigma-Aldrich H1270 Streptomycin sulfate salt Sigma-Aldrich S1277 Brain Heart Infusion (BHI) Sigma-Aldrich 53286 Trypticase Soy Broth Merck Millipore 22092 Yeast Extract Carl Roth 2363.2 Potassium dihydrogen phosphate (KH2PO4) Sigma-Aldrich P749.1 Glucose Merck Millipore 1.08337 Sodium carbonate (NA2CO3) VWR 27771 Mucin from procine stomach Sigma-Aldrich M2378 Cysteine-HCl Sigma-Aldrich C7477 Menadione Merck Millipore M5625 Hemin Merck Millipore 51280 Lipofectamine RNAiMAX ThermoFischer Scientific 13778075 Silencer Select Negative Control No. 1 siRNA ThermoFischer Scientific 4390843 eBioscience Cell Proliferation Dye eFluor 450 ThermoFischer Scientific 65-0842-85 Fast Green FCF dye Sigma-Aldrich F7252 TrypLE Express Enzyme (1X), no phenol red Gibco 12604021 Y-27632 (ROCK inhibitor) Tocris/Bio-Techne 1254 Matrigel Corning 356231 Azoxymethane Sigma-Aldrich A5486 Anaerobic Basal Broth Neolab M1636 Agar ThermoFischer Scientific 10572775 Horse Blood (Defibrinated, Sterile) TEBU BIO 007CL1500-50D Fluorescein isothiocyanate–dextran Sigma-Aldrich 46944 DTT Carl Roth 6908.2 Phorbol 12-myristate 13-acetate (PMA) Sigma-Aldrich P1585 Ionomycin ThermoFischer Scientific J62448MCR RPMI 1640 Medium, GlutaMAX Supplement Gibco 61870010 Erylysis-Buffer Morphisto 12972.00 Fluoromount-G Mounting Medium Invitrogen 00-4958-02 Brefeldin A Solution (1000X) Invitrogen 00-4506-51 Phenex-PTFE Syringe Filters Phenomenex AF0-3202-52 Advanced DMEM/F-12 ThermoFischer Scientific 12634028 B-27 Supplement (S50X), minus vitamin A ThermoFischer Scientific 12587010 GlutaMAX Supplement Gibco 35050061 HEPES (1M) ThermoFischer Scientific 15630056 N-Acetyl-L-cysteine Sigma-Aldrich A9165 Nicotinamide Sigma-Aldrich N0636 Noggin conditioned medium ImmunoPrecise N002 R-spondin I conditioned medium ImmunoPrecise R001 Human EGF Peprotech AF-100-15 A83-01 Tocris 2939 Prostaglandin E2 Tocris 2296 SB202190 Sigma-Aldrich S7076 Wnt surrogate U-Protein Express N001 Primocin InvivoGen ant-pm-05 Aqueous Glutaraldehyde EM Grade 25% Electron Microscopy Sciences 16220 Trihydrate Sodium Cacodylate Electron Microscopy Sciences 12310 (Continued on next page) ll OPEN ACCESS Article e3 Cell Host & Microbe 34, 1–17.e1–e12, November 11, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Osmium Tetroxide Aqueous Solution Electron Microscopy Sciences 19170 AGAR 100 Resin Agar Scientific R1031 Opti-MEM I Reduced Serum Medium ThermoFischer Scientific 31985062 Trypsin-EDTA (0.05%), phenol red ThermoFischer Scientific 25300062 Fetal Bovine Serum, Value ThermoFischer Scientific A5256701 GelGreen VWR 730–1535 Sodium chloride (NaCl) MP Biomedicals 194738 EDTA ThermoFischer Scientific 15575020 Tris Carl Roth 4855.3 Hydrochloric acid (HCl) Carl Roth 2607.1 DMSO ITW Reagents A3672,0050 Sodium hydroxide (NaOH) Carl Roth 6785.1 Eosin Y solution Carl Roth 229C.1 ROTIHistofix Carl Roth P087.5 Hematoxylin Solution, Gill No. 3 Sigma-Aldrich GHS316 Stealth siRNA ANXA2 ThermoFischer Scientific HSS179173 CAT#1299001 Critical commercial assays Opal 520 Reagent Pack Akoya Biosciences FP1487001KT Opal 480 Reagent Pack Akoya Biosciences FP1500001KT eBioscience Foxp3 / Transcription Factor Staining Buffer Set ThermoFischer Scientific 00-5523-00 LIVE/DEAD Fixable Near-IR Dead Cell Stain Kit Invitrogen L34975 CellTrace CFSE Cell Proliferation Kit, for flow cytometry ThermoFischer Scientific C34554 SignalStain DAB Substrate Kit CST 8059 Mouse IL-22 DuoSet ELISA R&D Systems DY582 Tumor Dissociation Kit, human Miltenyi Biotec 130-095-929 Lamina Propria Dissociation Kit, RNA Sequencing:Article Title: Gemella morbillorum promotes colorectal tumorigenesis through barrier disruption and invasion-dependent DNA damage. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER PitStop2 MedChemExpress HY-115604 Triton X-100 Sigma-Aldrich T8787 Horse Serum Sigma-Aldrich H1270 Streptomycin sulfate salt Sigma-Aldrich S1277 Brain Heart Infusion (BHI) Sigma-Aldrich 53286 Trypticase Soy Broth Merck Millipore 22092 Yeast Extract Carl Roth 2363.2 Potassium dihydrogen phosphate (KH2PO4) Sigma-Aldrich P749.1 Glucose Merck Millipore 1.08337 Sodium carbonate (NA2CO3) VWR 27771 Mucin from procine stomach Sigma-Aldrich M2378 Cysteine-HCl Sigma-Aldrich C7477 Menadione Merck Millipore M5625 Hemin Merck Millipore 51280 Lipofectamine RNAiMAX ThermoFischer Scientific 13778075 Silencer Select Negative Control No. 1 siRNA ThermoFischer Scientific 4390843 eBioscience Cell Proliferation Dye eFluor 450 ThermoFischer Scientific 65-0842-85 Fast Green FCF dye Sigma-Aldrich F7252 TrypLE Express Enzyme (1X), no phenol red Gibco 12604021 Y-27632 (ROCK inhibitor) Tocris/Bio-Techne 1254 Matrigel Corning 356231 Azoxymethane Sigma-Aldrich A5486 Anaerobic Basal Broth Neolab M1636 Agar ThermoFischer Scientific 10572775 Horse Blood (Defibrinated, Sterile) TEBU BIO 007CL1500-50D Fluorescein isothiocyanate–dextran Sigma-Aldrich 46944 DTT Carl Roth 6908.2 Phorbol 12-myristate 13-acetate (PMA) Sigma-Aldrich P1585 Ionomycin ThermoFischer Scientific J62448MCR RPMI 1640 Medium, GlutaMAX Supplement Gibco 61870010 Erylysis-Buffer Morphisto 12972.00 Fluoromount-G Mounting Medium Invitrogen 00-4958-02 Brefeldin A Solution (1000X) Invitrogen 00-4506-51 Phenex-PTFE Syringe Filters Phenomenex AF0-3202-52 Advanced DMEM/F-12 ThermoFischer Scientific 12634028 B-27 Supplement (S50X), minus vitamin A ThermoFischer Scientific 12587010 GlutaMAX Supplement Gibco 35050061 HEPES (1M) ThermoFischer Scientific 15630056 N-Acetyl-L-cysteine Sigma-Aldrich A9165 Nicotinamide Sigma-Aldrich N0636 Noggin conditioned medium ImmunoPrecise N002 R-spondin I conditioned medium ImmunoPrecise R001 Human EGF Peprotech AF-100-15 A83-01 Tocris 2939 Prostaglandin E2 Tocris 2296 SB202190 Sigma-Aldrich S7076 Wnt surrogate U-Protein Express N001 Primocin InvivoGen ant-pm-05 Aqueous Glutaraldehyde EM Grade 25% Electron Microscopy Sciences 16220 Trihydrate Sodium Cacodylate Electron Microscopy Sciences 12310 (Continued on next page) ll OPEN ACCESS Article e3 Cell Host & Microbe 34, 1–17.e1–e12, November 11, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Osmium Tetroxide Aqueous Solution Electron Microscopy Sciences 19170 AGAR 100 Resin Agar Scientific R1031 Opti-MEM I Reduced Serum Medium ThermoFischer Scientific 31985062 Trypsin-EDTA (0.05%), phenol red ThermoFischer Scientific 25300062 Fetal Bovine Serum, Value ThermoFischer Scientific A5256701 GelGreen VWR 730–1535 Sodium chloride (NaCl) MP Biomedicals 194738 EDTA ThermoFischer Scientific 15575020 Tris Carl Roth 4855.3 Hydrochloric acid (HCl) Carl Roth 2607.1 DMSO ITW Reagents A3672,0050 Sodium hydroxide (NaOH) Carl Roth 6785.1 Eosin Y solution Carl Roth 229C.1 ROTIHistofix Carl Roth P087.5 Hematoxylin Solution, Gill No. 3 Sigma-Aldrich GHS316 Stealth siRNA ANXA2 ThermoFischer Scientific HSS179173 CAT#1299001 Critical commercial assays Opal 520 Reagent Pack Akoya Biosciences FP1487001KT Opal 480 Reagent Pack Akoya Biosciences FP1500001KT eBioscience Foxp3 / Transcription Factor Staining Buffer Set ThermoFischer Scientific 00-5523-00 LIVE/DEAD Fixable Near-IR Dead Cell Stain Kit Invitrogen L34975 CellTrace CFSE Cell Proliferation Kit, for flow cytometry ThermoFischer Scientific C34554 SignalStain DAB Substrate Kit CST 8059 Mouse IL-22 DuoSet ELISA R&D Systems DY582 Tumor Dissociation Kit, human Miltenyi Biotec 130-095-929 Lamina Propria Dissociation Kit, Analysis:Article Title: Gemella morbillorum promotes colorectal tumorigenesis through barrier disruption and invasion-dependent DNA damage. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER PitStop2 MedChemExpress HY-115604 Triton X-100 Sigma-Aldrich T8787 Horse Serum Sigma-Aldrich H1270 Streptomycin sulfate salt Sigma-Aldrich S1277 Brain Heart Infusion (BHI) Sigma-Aldrich 53286 Trypticase Soy Broth Merck Millipore 22092 Yeast Extract Carl Roth 2363.2 Potassium dihydrogen phosphate (KH2PO4) Sigma-Aldrich P749.1 Glucose Merck Millipore 1.08337 Sodium carbonate (NA2CO3) VWR 27771 Mucin from procine stomach Sigma-Aldrich M2378 Cysteine-HCl Sigma-Aldrich C7477 Menadione Merck Millipore M5625 Hemin Merck Millipore 51280 Lipofectamine RNAiMAX ThermoFischer Scientific 13778075 Silencer Select Negative Control No. 1 siRNA ThermoFischer Scientific 4390843 eBioscience Cell Proliferation Dye eFluor 450 ThermoFischer Scientific 65-0842-85 Fast Green FCF dye Sigma-Aldrich F7252 TrypLE Express Enzyme (1X), no phenol red Gibco 12604021 Y-27632 (ROCK inhibitor) Tocris/Bio-Techne 1254 Matrigel Corning 356231 Azoxymethane Sigma-Aldrich A5486 Anaerobic Basal Broth Neolab M1636 Agar ThermoFischer Scientific 10572775 Horse Blood (Defibrinated, Sterile) TEBU BIO 007CL1500-50D Fluorescein isothiocyanate–dextran Sigma-Aldrich 46944 DTT Carl Roth 6908.2 Phorbol 12-myristate 13-acetate (PMA) Sigma-Aldrich P1585 Ionomycin ThermoFischer Scientific J62448MCR RPMI 1640 Medium, GlutaMAX Supplement Gibco 61870010 Erylysis-Buffer Morphisto 12972.00 Fluoromount-G Mounting Medium Invitrogen 00-4958-02 Brefeldin A Solution (1000X) Invitrogen 00-4506-51 Phenex-PTFE Syringe Filters Phenomenex AF0-3202-52 Advanced DMEM/F-12 ThermoFischer Scientific 12634028 B-27 Supplement (S50X), minus vitamin A ThermoFischer Scientific 12587010 GlutaMAX Supplement Gibco 35050061 HEPES (1M) ThermoFischer Scientific 15630056 N-Acetyl-L-cysteine Sigma-Aldrich A9165 Nicotinamide Sigma-Aldrich N0636 Noggin conditioned medium ImmunoPrecise N002 R-spondin I conditioned medium ImmunoPrecise R001 Human EGF Peprotech AF-100-15 A83-01 Tocris 2939 Prostaglandin E2 Tocris 2296 SB202190 Sigma-Aldrich S7076 Wnt surrogate U-Protein Express N001 Primocin InvivoGen ant-pm-05 Aqueous Glutaraldehyde EM Grade 25% Electron Microscopy Sciences 16220 Trihydrate Sodium Cacodylate Electron Microscopy Sciences 12310 (Continued on next page) ll OPEN ACCESS Article e3 Cell Host & Microbe 34, 1–17.e1–e12, November 11, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Osmium Tetroxide Aqueous Solution Electron Microscopy Sciences 19170 AGAR 100 Resin Agar Scientific R1031 Opti-MEM I Reduced Serum Medium ThermoFischer Scientific 31985062 Trypsin-EDTA (0.05%), phenol red ThermoFischer Scientific 25300062 Fetal Bovine Serum, Value ThermoFischer Scientific A5256701 GelGreen VWR 730–1535 Sodium chloride (NaCl) MP Biomedicals 194738 EDTA ThermoFischer Scientific 15575020 Tris Carl Roth 4855.3 Hydrochloric acid (HCl) Carl Roth 2607.1 DMSO ITW Reagents A3672,0050 Sodium hydroxide (NaOH) Carl Roth 6785.1 Eosin Y solution Carl Roth 229C.1 ROTIHistofix Carl Roth P087.5 Hematoxylin Solution, Gill No. 3 Sigma-Aldrich GHS316 Stealth siRNA ANXA2 ThermoFischer Scientific HSS179173 CAT#1299001 Critical commercial assays Opal 520 Reagent Pack Akoya Biosciences FP1487001KT Opal 480 Reagent Pack Akoya Biosciences FP1500001KT eBioscience Foxp3 / Transcription Factor Staining Buffer Set ThermoFischer Scientific 00-5523-00 LIVE/DEAD Fixable Near-IR Dead Cell Stain Kit Invitrogen L34975 CellTrace CFSE Cell Proliferation Kit, for flow cytometry ThermoFischer Scientific C34554 SignalStain DAB Substrate Kit CST 8059 Mouse IL-22 DuoSet ELISA R&D Systems DY582 Tumor Dissociation Kit, human Miltenyi Biotec 130-095-929 Lamina Propria Dissociation Kit, Lipidomics:Article Title: Gemella morbillorum promotes colorectal tumorigenesis through barrier disruption and invasion-dependent DNA damage. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER PitStop2 MedChemExpress HY-115604 Triton X-100 Sigma-Aldrich T8787 Horse Serum Sigma-Aldrich H1270 Streptomycin sulfate salt Sigma-Aldrich S1277 Brain Heart Infusion (BHI) Sigma-Aldrich 53286 Trypticase Soy Broth Merck Millipore 22092 Yeast Extract Carl Roth 2363.2 Potassium dihydrogen phosphate (KH2PO4) Sigma-Aldrich P749.1 Glucose Merck Millipore 1.08337 Sodium carbonate (NA2CO3) VWR 27771 Mucin from procine stomach Sigma-Aldrich M2378 Cysteine-HCl Sigma-Aldrich C7477 Menadione Merck Millipore M5625 Hemin Merck Millipore 51280 Lipofectamine RNAiMAX ThermoFischer Scientific 13778075 Silencer Select Negative Control No. 1 siRNA ThermoFischer Scientific 4390843 eBioscience Cell Proliferation Dye eFluor 450 ThermoFischer Scientific 65-0842-85 Fast Green FCF dye Sigma-Aldrich F7252 TrypLE Express Enzyme (1X), no phenol red Gibco 12604021 Y-27632 (ROCK inhibitor) Tocris/Bio-Techne 1254 Matrigel Corning 356231 Azoxymethane Sigma-Aldrich A5486 Anaerobic Basal Broth Neolab M1636 Agar ThermoFischer Scientific 10572775 Horse Blood (Defibrinated, Sterile) TEBU BIO 007CL1500-50D Fluorescein isothiocyanate–dextran Sigma-Aldrich 46944 DTT Carl Roth 6908.2 Phorbol 12-myristate 13-acetate (PMA) Sigma-Aldrich P1585 Ionomycin ThermoFischer Scientific J62448MCR RPMI 1640 Medium, GlutaMAX Supplement Gibco 61870010 Erylysis-Buffer Morphisto 12972.00 Fluoromount-G Mounting Medium Invitrogen 00-4958-02 Brefeldin A Solution (1000X) Invitrogen 00-4506-51 Phenex-PTFE Syringe Filters Phenomenex AF0-3202-52 Advanced DMEM/F-12 ThermoFischer Scientific 12634028 B-27 Supplement (S50X), minus vitamin A ThermoFischer Scientific 12587010 GlutaMAX Supplement Gibco 35050061 HEPES (1M) ThermoFischer Scientific 15630056 N-Acetyl-L-cysteine Sigma-Aldrich A9165 Nicotinamide Sigma-Aldrich N0636 Noggin conditioned medium ImmunoPrecise N002 R-spondin I conditioned medium ImmunoPrecise R001 Human EGF Peprotech AF-100-15 A83-01 Tocris 2939 Prostaglandin E2 Tocris 2296 SB202190 Sigma-Aldrich S7076 Wnt surrogate U-Protein Express N001 Primocin InvivoGen ant-pm-05 Aqueous Glutaraldehyde EM Grade 25% Electron Microscopy Sciences 16220 Trihydrate Sodium Cacodylate Electron Microscopy Sciences 12310 (Continued on next page) ll OPEN ACCESS Article e3 Cell Host & Microbe 34, 1–17.e1–e12, November 11, 2026 .. REAGENT or RESOURCE SOURCE IDENTIFIER Osmium Tetroxide Aqueous Solution Electron Microscopy Sciences 19170 AGAR 100 Resin Agar Scientific R1031 Opti-MEM I Reduced Serum Medium ThermoFischer Scientific 31985062 Trypsin-EDTA (0.05%), phenol red ThermoFischer Scientific 25300062 Fetal Bovine Serum, Value ThermoFischer Scientific A5256701 GelGreen VWR 730–1535 Sodium chloride (NaCl) MP Biomedicals 194738 EDTA ThermoFischer Scientific 15575020 Tris Carl Roth 4855.3 Hydrochloric acid (HCl) Carl Roth 2607.1 DMSO ITW Reagents A3672,0050 Sodium hydroxide (NaOH) Carl Roth 6785.1 Eosin Y solution Carl Roth 229C.1 ROTIHistofix Carl Roth P087.5 Hematoxylin Solution, Gill No. 3 Sigma-Aldrich GHS316 Stealth siRNA ANXA2 ThermoFischer Scientific HSS179173 CAT#1299001 Critical commercial assays Opal 520 Reagent Pack Akoya Biosciences FP1487001KT Opal 480 Reagent Pack Akoya Biosciences FP1500001KT eBioscience Foxp3 / Transcription Factor Staining Buffer Set ThermoFischer Scientific 00-5523-00 LIVE/DEAD Fixable Near-IR Dead Cell Stain Kit Invitrogen L34975 CellTrace CFSE Cell Proliferation Kit, for flow cytometry ThermoFischer Scientific C34554 SignalStain DAB Substrate Kit CST 8059 Mouse IL-22 DuoSet ELISA R&D Systems DY582 Tumor Dissociation Kit, human Miltenyi Biotec 130-095-929 Lamina Propria Dissociation Kit, Virus:Article Title: Perinatal nicotine exposure disrupts hematopoietic stem cell development and elevates influenza severity in adulthood. Article Snippet: .. Biotin anti-mouse CD34 Antibody eBioscience 13-0341-85 APC anti-mouse CD135 Antibody Biolegend 135310; RRID: AB_2107050 PE/Cyanine5 anti-mouse TER-119/Erythroid Cells Antibody BioLegend 116210; RRID: AB_313711 Alexa Fluor 647 anti-mouse/rat CD61 Antibody Biolegend 104313; RRID: AB_2249337 Pacific Blue anti-mouse Ly-6G/Ly-6C (Gr-1) Antibody BioLegend 108430; RRID: AB_893556 Biotin anti-mouse CD71 (Transferrin Receptor) Antibody Invitrogen 13-0711-82 PE/Cyanine7 anti-mouse/human CD11b Antibody BioLegend 101216; RRID: AB_312799 APC anti-mouse CD3ε Antibody BioLegend 100312; RRID: AB_312677 Brilliant Violet 605 anti-mouse CD4 Antibody BioLegend 100451; RRID: AB_2564591 APC/Cyanine7 anti-mouse CD8a Antibody BioLegend 100714; RRID: AB_11124344 PE/Cyanine7 anti-mouse TCR β chain Antibody BioLegend 109222; RRID: AB_893625 Brilliant Violet 421 anti-mouse TCR γ/δ Antibody BioLegend 118120; RRID: AB_2562566 Brilliant Violet 785 anti-mouse CD19 Antibody BioLegend 115543; RRID: AB_11218994 Alexa Fluor 647 anti-mouse CD5 Antibody BioLegend 100614; RRID: AB_2075301 PE/Cyanine7 anti-mouse CD21/CD35 Antibody Invitrogen 25-0211-82 Pacific Blue anti-mouse CD23 Antibody BioLegend 101616; RRID: AB_2103306 Brilliant Violet 785 anti-mouse Siglec-F Antibody Waters Biosciences 740956 APC/Cyanine7 anti-mouse CD11c Antibody BioLegend 117324; RRID: AB_830649 APC anti-mouse Ly-6G Antibody BioLegend 127614; RRID: AB_2227348 Brilliant Violet 605 anti-mouse Ly-6G/Ly6C (Gr-1) Antibody BioLegend 108440; RRID: AB_2563311 Brilliant Violet 510 anti-mouse CD71 Antibody BioLegend 113823; RRID: AB_2783053 APC/Cyanine7 anti-mouse CD45 Antibody BioLegend 103116; RRID: AB_312981 PE/Cyanine7 anti-mouse CD41 Antibody BioLegend 133916; RRID: AB_11124102 Pacific Blue anti-mouse NK-1.1 Antibody BioLegend 108722; RRID: AB_2132712 Alexa Fluor 700 anti-mouse CD48 Antibody BioLegend 103426; RRID: AB_10612755 CD117 MicroBeads, Article Title: Replaying germinal center evolution on a quantified affinity landscape. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-CD16/32 (Fc block) (clone 2.4G2) BD Biosciences RRID: AB_394656 Anti-B220 BV785 (clone RA3-6B2) Biolegend RRID: AB_11218795 Anti-CD38 APC (clone 90) Invitrogen RRID: AB_469382 Anti-CD95 (Fas) Pe-Cy7 (clone Jo2) BD Biosciences RRID: AB_396768 Streptavidin BV421 Biolegend Cat# 405226 Anti-Igκ PE (clone 187.1) BD Biosciences Cat# 559940; RRID:AB_397384 Streptavidin PE Thermo Fisher Scientific Cat# S866 Anti-TCRβ APC-eFluor780 (clone H57–597) eBioscience RRID: AB_1272173 Anti-c-Myc FITC (CYMC-45F) Immunology Consultants Lab Cat# CYMC-45F Anti-CD35 Cy3 (clone 8C12) In house N/A Anti-GFP BioLegend Cat# 902605; RRID: AB_2734671 TotalSeqTM-C0307 anti-mouse Hashtag Antibody (1–16) BioLegend Various Bacterial and virus strains E. Coli New England Biolabs Cat# C3020K Biological samples Plasmodium Chabaudi BEI Resources Cat# MRA-741 Chemicals, peptides, and recombinant proteins Imject Alum Thermo Fisher Scientific Cat# 77161 Alhydrogel adjuvant 2% InvivoGen Cat# vac-alu-250 Normal Chicken IgY (egg-derived) Exalpha Biologicals Cat# IgY-100 ACK lysing buffer Thermo Fisher Scientific Cat# A10492-01 Tween20 Sigma-Aldrich Cat# P9416-50ML Bovine Serum Albumin (BSA) Sigma-Aldrich Cat# A9647-500G Fetal bovine serum (FBS) Thermo Fisher Scientific Cat# A3160401 Buffer TCL Qiagen Cat# 1031576 EDTA Spectrum Laboratories Cat# 40520000-3 Trizol LS Thermo Fisher Scientific Cat# 10296010 Beta mercaptoethanol Thermo Fisher Scientific Cat #21985023 ECL substrate Pierce N/A Critical commercial assays Zeba desalting column purification Thermo Fisher Scientific Cat# 89883 Protein-G Sepharose Fisher Scientific Cat# 45-000-116 Ni-INDIGO Agarose Beads PureCube Cat# 75105 MACS Cell Separation Column LS Miltenyi Biotec Cat# 130-042-401 CD43 (Ly-48) microbeads, Stem Cell:Article Title: Perinatal nicotine exposure disrupts hematopoietic stem cell development and elevates influenza severity in adulthood. Article Snippet: .. Biotin anti-mouse CD34 Antibody eBioscience 13-0341-85 APC anti-mouse CD135 Antibody Biolegend 135310; RRID: AB_2107050 PE/Cyanine5 anti-mouse TER-119/Erythroid Cells Antibody BioLegend 116210; RRID: AB_313711 Alexa Fluor 647 anti-mouse/rat CD61 Antibody Biolegend 104313; RRID: AB_2249337 Pacific Blue anti-mouse Ly-6G/Ly-6C (Gr-1) Antibody BioLegend 108430; RRID: AB_893556 Biotin anti-mouse CD71 (Transferrin Receptor) Antibody Invitrogen 13-0711-82 PE/Cyanine7 anti-mouse/human CD11b Antibody BioLegend 101216; RRID: AB_312799 APC anti-mouse CD3ε Antibody BioLegend 100312; RRID: AB_312677 Brilliant Violet 605 anti-mouse CD4 Antibody BioLegend 100451; RRID: AB_2564591 APC/Cyanine7 anti-mouse CD8a Antibody BioLegend 100714; RRID: AB_11124344 PE/Cyanine7 anti-mouse TCR β chain Antibody BioLegend 109222; RRID: AB_893625 Brilliant Violet 421 anti-mouse TCR γ/δ Antibody BioLegend 118120; RRID: AB_2562566 Brilliant Violet 785 anti-mouse CD19 Antibody BioLegend 115543; RRID: AB_11218994 Alexa Fluor 647 anti-mouse CD5 Antibody BioLegend 100614; RRID: AB_2075301 PE/Cyanine7 anti-mouse CD21/CD35 Antibody Invitrogen 25-0211-82 Pacific Blue anti-mouse CD23 Antibody BioLegend 101616; RRID: AB_2103306 Brilliant Violet 785 anti-mouse Siglec-F Antibody Waters Biosciences 740956 APC/Cyanine7 anti-mouse CD11c Antibody BioLegend 117324; RRID: AB_830649 APC anti-mouse Ly-6G Antibody BioLegend 127614; RRID: AB_2227348 Brilliant Violet 605 anti-mouse Ly-6G/Ly6C (Gr-1) Antibody BioLegend 108440; RRID: AB_2563311 Brilliant Violet 510 anti-mouse CD71 Antibody BioLegend 113823; RRID: AB_2783053 APC/Cyanine7 anti-mouse CD45 Antibody BioLegend 103116; RRID: AB_312981 PE/Cyanine7 anti-mouse CD41 Antibody BioLegend 133916; RRID: AB_11124102 Pacific Blue anti-mouse NK-1.1 Antibody BioLegend 108722; RRID: AB_2132712 Alexa Fluor 700 anti-mouse CD48 Antibody BioLegend 103426; RRID: AB_10612755 CD117 MicroBeads, Reverse Transcription:Article Title: Resident mesenchymal progenitor cells require autocrine IGF-I in homeostatic and regenerating skeletal muscle. Article Snippet: .. Compound Sakura Cat#4583 2-methylbutane Sigma Cat#277258-1L Trizol Reagent Thermo Fisher Cat#15596026 DNase I Thermo Fisher Cat#18047019 High Capacity cDNA Reverse Transcription Kit Thermo Fisher Cat#4368814 SYBR Green Master Mix Standard Thermo Fisher Cat#A46109 RNeasy Micro Kit Qiagen Cat#74004 Fluoromount-G Southern Biotech Cat#0100-01 Pierce BCA Protein Assay Thermo Fisher Cat#23227 NuPAGE Bis-Tris Mini Gel Thermo Fisher Cat#10780345 Intercept Blocking Buffer Licor Cat#927-60001 Debris Removal Solution Miltenyi Biotec Cat#130-109-398 PDGFRa Microbead Kits Miltenyi Biotec Cat#130-101-502 Satellite Cell isolation Kit, SYBR Green Assay:Article Title: Resident mesenchymal progenitor cells require autocrine IGF-I in homeostatic and regenerating skeletal muscle. Article Snippet: .. Compound Sakura Cat#4583 2-methylbutane Sigma Cat#277258-1L Trizol Reagent Thermo Fisher Cat#15596026 DNase I Thermo Fisher Cat#18047019 High Capacity cDNA Reverse Transcription Kit Thermo Fisher Cat#4368814 SYBR Green Master Mix Standard Thermo Fisher Cat#A46109 RNeasy Micro Kit Qiagen Cat#74004 Fluoromount-G Southern Biotech Cat#0100-01 Pierce BCA Protein Assay Thermo Fisher Cat#23227 NuPAGE Bis-Tris Mini Gel Thermo Fisher Cat#10780345 Intercept Blocking Buffer Licor Cat#927-60001 Debris Removal Solution Miltenyi Biotec Cat#130-109-398 PDGFRa Microbead Kits Miltenyi Biotec Cat#130-101-502 Satellite Cell isolation Kit, Bicinchoninic Acid Protein Assay:Article Title: Resident mesenchymal progenitor cells require autocrine IGF-I in homeostatic and regenerating skeletal muscle. Article Snippet: .. Compound Sakura Cat#4583 2-methylbutane Sigma Cat#277258-1L Trizol Reagent Thermo Fisher Cat#15596026 DNase I Thermo Fisher Cat#18047019 High Capacity cDNA Reverse Transcription Kit Thermo Fisher Cat#4368814 SYBR Green Master Mix Standard Thermo Fisher Cat#A46109 RNeasy Micro Kit Qiagen Cat#74004 Fluoromount-G Southern Biotech Cat#0100-01 Pierce BCA Protein Assay Thermo Fisher Cat#23227 NuPAGE Bis-Tris Mini Gel Thermo Fisher Cat#10780345 Intercept Blocking Buffer Licor Cat#927-60001 Debris Removal Solution Miltenyi Biotec Cat#130-109-398 PDGFRa Microbead Kits Miltenyi Biotec Cat#130-101-502 Satellite Cell isolation Kit, Blocking Assay:Article Title: Resident mesenchymal progenitor cells require autocrine IGF-I in homeostatic and regenerating skeletal muscle. Article Snippet: .. Compound Sakura Cat#4583 2-methylbutane Sigma Cat#277258-1L Trizol Reagent Thermo Fisher Cat#15596026 DNase I Thermo Fisher Cat#18047019 High Capacity cDNA Reverse Transcription Kit Thermo Fisher Cat#4368814 SYBR Green Master Mix Standard Thermo Fisher Cat#A46109 RNeasy Micro Kit Qiagen Cat#74004 Fluoromount-G Southern Biotech Cat#0100-01 Pierce BCA Protein Assay Thermo Fisher Cat#23227 NuPAGE Bis-Tris Mini Gel Thermo Fisher Cat#10780345 Intercept Blocking Buffer Licor Cat#927-60001 Debris Removal Solution Miltenyi Biotec Cat#130-109-398 PDGFRa Microbead Kits Miltenyi Biotec Cat#130-101-502 Satellite Cell isolation Kit, Article Title: Replaying germinal center evolution on a quantified affinity landscape. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-CD16/32 (Fc block) (clone 2.4G2) BD Biosciences RRID: AB_394656 Anti-B220 BV785 (clone RA3-6B2) Biolegend RRID: AB_11218795 Anti-CD38 APC (clone 90) Invitrogen RRID: AB_469382 Anti-CD95 (Fas) Pe-Cy7 (clone Jo2) BD Biosciences RRID: AB_396768 Streptavidin BV421 Biolegend Cat# 405226 Anti-Igκ PE (clone 187.1) BD Biosciences Cat# 559940; RRID:AB_397384 Streptavidin PE Thermo Fisher Scientific Cat# S866 Anti-TCRβ APC-eFluor780 (clone H57–597) eBioscience RRID: AB_1272173 Anti-c-Myc FITC (CYMC-45F) Immunology Consultants Lab Cat# CYMC-45F Anti-CD35 Cy3 (clone 8C12) In house N/A Anti-GFP BioLegend Cat# 902605; RRID: AB_2734671 TotalSeqTM-C0307 anti-mouse Hashtag Antibody (1–16) BioLegend Various Bacterial and virus strains E. Coli New England Biolabs Cat# C3020K Biological samples Plasmodium Chabaudi BEI Resources Cat# MRA-741 Chemicals, peptides, and recombinant proteins Imject Alum Thermo Fisher Scientific Cat# 77161 Alhydrogel adjuvant 2% InvivoGen Cat# vac-alu-250 Normal Chicken IgY (egg-derived) Exalpha Biologicals Cat# IgY-100 ACK lysing buffer Thermo Fisher Scientific Cat# A10492-01 Tween20 Sigma-Aldrich Cat# P9416-50ML Bovine Serum Albumin (BSA) Sigma-Aldrich Cat# A9647-500G Fetal bovine serum (FBS) Thermo Fisher Scientific Cat# A3160401 Buffer TCL Qiagen Cat# 1031576 EDTA Spectrum Laboratories Cat# 40520000-3 Trizol LS Thermo Fisher Scientific Cat# 10296010 Beta mercaptoethanol Thermo Fisher Scientific Cat #21985023 ECL substrate Pierce N/A Critical commercial assays Zeba desalting column purification Thermo Fisher Scientific Cat# 89883 Protein-G Sepharose Fisher Scientific Cat# 45-000-116 Ni-INDIGO Agarose Beads PureCube Cat# 75105 MACS Cell Separation Column LS Miltenyi Biotec Cat# 130-042-401 CD43 (Ly-48) microbeads, Cell Isolation:Article Title: Resident mesenchymal progenitor cells require autocrine IGF-I in homeostatic and regenerating skeletal muscle. Article Snippet: .. Compound Sakura Cat#4583 2-methylbutane Sigma Cat#277258-1L Trizol Reagent Thermo Fisher Cat#15596026 DNase I Thermo Fisher Cat#18047019 High Capacity cDNA Reverse Transcription Kit Thermo Fisher Cat#4368814 SYBR Green Master Mix Standard Thermo Fisher Cat#A46109 RNeasy Micro Kit Qiagen Cat#74004 Fluoromount-G Southern Biotech Cat#0100-01 Pierce BCA Protein Assay Thermo Fisher Cat#23227 NuPAGE Bis-Tris Mini Gel Thermo Fisher Cat#10780345 Intercept Blocking Buffer Licor Cat#927-60001 Debris Removal Solution Miltenyi Biotec Cat#130-109-398 PDGFRa Microbead Kits Miltenyi Biotec Cat#130-101-502 Satellite Cell isolation Kit, Membrane:Article Title: Resident mesenchymal progenitor cells require autocrine IGF-I in homeostatic and regenerating skeletal muscle. Article Snippet: .. Compound Sakura Cat#4583 2-methylbutane Sigma Cat#277258-1L Trizol Reagent Thermo Fisher Cat#15596026 DNase I Thermo Fisher Cat#18047019 High Capacity cDNA Reverse Transcription Kit Thermo Fisher Cat#4368814 SYBR Green Master Mix Standard Thermo Fisher Cat#A46109 RNeasy Micro Kit Qiagen Cat#74004 Fluoromount-G Southern Biotech Cat#0100-01 Pierce BCA Protein Assay Thermo Fisher Cat#23227 NuPAGE Bis-Tris Mini Gel Thermo Fisher Cat#10780345 Intercept Blocking Buffer Licor Cat#927-60001 Debris Removal Solution Miltenyi Biotec Cat#130-109-398 PDGFRa Microbead Kits Miltenyi Biotec Cat#130-101-502 Satellite Cell isolation Kit, Adjuvant:Article Title: Replaying germinal center evolution on a quantified affinity landscape. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-CD16/32 (Fc block) (clone 2.4G2) BD Biosciences RRID: AB_394656 Anti-B220 BV785 (clone RA3-6B2) Biolegend RRID: AB_11218795 Anti-CD38 APC (clone 90) Invitrogen RRID: AB_469382 Anti-CD95 (Fas) Pe-Cy7 (clone Jo2) BD Biosciences RRID: AB_396768 Streptavidin BV421 Biolegend Cat# 405226 Anti-Igκ PE (clone 187.1) BD Biosciences Cat# 559940; RRID:AB_397384 Streptavidin PE Thermo Fisher Scientific Cat# S866 Anti-TCRβ APC-eFluor780 (clone H57–597) eBioscience RRID: AB_1272173 Anti-c-Myc FITC (CYMC-45F) Immunology Consultants Lab Cat# CYMC-45F Anti-CD35 Cy3 (clone 8C12) In house N/A Anti-GFP BioLegend Cat# 902605; RRID: AB_2734671 TotalSeqTM-C0307 anti-mouse Hashtag Antibody (1–16) BioLegend Various Bacterial and virus strains E. Coli New England Biolabs Cat# C3020K Biological samples Plasmodium Chabaudi BEI Resources Cat# MRA-741 Chemicals, peptides, and recombinant proteins Imject Alum Thermo Fisher Scientific Cat# 77161 Alhydrogel adjuvant 2% InvivoGen Cat# vac-alu-250 Normal Chicken IgY (egg-derived) Exalpha Biologicals Cat# IgY-100 ACK lysing buffer Thermo Fisher Scientific Cat# A10492-01 Tween20 Sigma-Aldrich Cat# P9416-50ML Bovine Serum Albumin (BSA) Sigma-Aldrich Cat# A9647-500G Fetal bovine serum (FBS) Thermo Fisher Scientific Cat# A3160401 Buffer TCL Qiagen Cat# 1031576 EDTA Spectrum Laboratories Cat# 40520000-3 Trizol LS Thermo Fisher Scientific Cat# 10296010 Beta mercaptoethanol Thermo Fisher Scientific Cat #21985023 ECL substrate Pierce N/A Critical commercial assays Zeba desalting column purification Thermo Fisher Scientific Cat# 89883 Protein-G Sepharose Fisher Scientific Cat# 45-000-116 Ni-INDIGO Agarose Beads PureCube Cat# 75105 MACS Cell Separation Column LS Miltenyi Biotec Cat# 130-042-401 CD43 (Ly-48) microbeads, Purification:Article Title: Replaying germinal center evolution on a quantified affinity landscape. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-CD16/32 (Fc block) (clone 2.4G2) BD Biosciences RRID: AB_394656 Anti-B220 BV785 (clone RA3-6B2) Biolegend RRID: AB_11218795 Anti-CD38 APC (clone 90) Invitrogen RRID: AB_469382 Anti-CD95 (Fas) Pe-Cy7 (clone Jo2) BD Biosciences RRID: AB_396768 Streptavidin BV421 Biolegend Cat# 405226 Anti-Igκ PE (clone 187.1) BD Biosciences Cat# 559940; RRID:AB_397384 Streptavidin PE Thermo Fisher Scientific Cat# S866 Anti-TCRβ APC-eFluor780 (clone H57–597) eBioscience RRID: AB_1272173 Anti-c-Myc FITC (CYMC-45F) Immunology Consultants Lab Cat# CYMC-45F Anti-CD35 Cy3 (clone 8C12) In house N/A Anti-GFP BioLegend Cat# 902605; RRID: AB_2734671 TotalSeqTM-C0307 anti-mouse Hashtag Antibody (1–16) BioLegend Various Bacterial and virus strains E. Coli New England Biolabs Cat# C3020K Biological samples Plasmodium Chabaudi BEI Resources Cat# MRA-741 Chemicals, peptides, and recombinant proteins Imject Alum Thermo Fisher Scientific Cat# 77161 Alhydrogel adjuvant 2% InvivoGen Cat# vac-alu-250 Normal Chicken IgY (egg-derived) Exalpha Biologicals Cat# IgY-100 ACK lysing buffer Thermo Fisher Scientific Cat# A10492-01 Tween20 Sigma-Aldrich Cat# P9416-50ML Bovine Serum Albumin (BSA) Sigma-Aldrich Cat# A9647-500G Fetal bovine serum (FBS) Thermo Fisher Scientific Cat# A3160401 Buffer TCL Qiagen Cat# 1031576 EDTA Spectrum Laboratories Cat# 40520000-3 Trizol LS Thermo Fisher Scientific Cat# 10296010 Beta mercaptoethanol Thermo Fisher Scientific Cat #21985023 ECL substrate Pierce N/A Critical commercial assays Zeba desalting column purification Thermo Fisher Scientific Cat# 89883 Protein-G Sepharose Fisher Scientific Cat# 45-000-116 Ni-INDIGO Agarose Beads PureCube Cat# 75105 MACS Cell Separation Column LS Miltenyi Biotec Cat# 130-042-401 CD43 (Ly-48) microbeads, other:Article Title: Circulating memory-phenotype CD4 + T cells rapidly accumulate in the ischemic gut to exacerbate tissue injury in an innate manner. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Ionomycin Fujifilm Wako Cat# 091-05833 Brefeldin A Solution Biolegend Cat# 420601 Zombie UV Fixable Viability Kit Biolegend Cat# 423108 Foxp3/Transcription Factor Staining Buffer Set Thermo Fisher Scientific Cat# 00-5523-00 Nuclease-Free Water (not DEPC-Treated) Thermo Fisher Scientific Cat# AM9937 RNase Inhibitor Thermo Fisher Scientific Cat# N8080119 Mouse MOG(35–55) MEVGWYRSPFSRVVHLYRNGK PH Japan NA Bottle M Tuberculosis H37Ra Dessicated BD Biosciences Cat# 231141 Formaldehyde Solution Fujifilm Wako Cat# 061-00411 THUNDERBIRD SYBR qPCR Mix TOYOBO Cat# QPS-201 Dako Target Retrieval Solution Agilent Cat# S1699 HISTOFINE Simple Stain Mouse MAX-PO (Rat) Nichirei Biosciences Cat# 414311 HISTOFINE Simple Stain Mouse MAX-PO (Rabbit) Nichirei Biosciences Cat# 414341 3, 3′-diaminobenzidine tetrahydrochloride Dojindo Laboratories Cat# 349-00903 Histofine Antigen Retrieval Solution (pH 9.0) Nichirei Biosciences Cat# 415211 Histogreen Biozol Cat# E109 O.C.T. compound Sakura Finetek Japan Cat# 4583 Percoll Cytiva Cat# 17089101 Critical commercial assays Lamina Propria Dissociation Kit, Article Title: Lipid oxidation reprogramming in cancer-associated fibroblasts enhances CD8 + T cell cytotoxicity and therapeutic response. Article Snippet: Article Lipid oxidation reprogramming in cancer-associated fibroblasts enhances CD8+ T cell cytotoxicity and therapeutic response Western Blot:Article Title: Protocol for minimized-bias profiling of liver and visceral adipose tissue in mice using integrated single-nucleus RNA sequencing. Article Snippet: .. Biological samples Male wild-type C57BL/6J mice, 8 weeks old at diet initiation; tissues collected after 26 weeks of western diet feeding Janvier Labs Ethics approval #47097-2024012516265646 v4 Chemicals, peptides, and recombinant proteins PBS Gibco Cat#10010023 DMEM Gibco Cat#10566016 Absolute ethanol Major supplier N/A Nuclease-free water Major supplier N/A DAPI Staining Solution MILTENYI BIOTEC Cat#130-111-570 RNase inhibitor, 40000 U/mL New England Biolabs Cat#M0314 BD Stain Buffer (FBS) BD Biosciences Cat#554656 Proteinase K, 800 U/mL New England Biolabs Cat#P8107S Vybrant® DyeCycleTM Green Stain Thermo Fisher Cat#V35004 Critical commercial assays Nuclei Extraction Buffer MILTENYI BIOTEC Cat#130-128-024 Liver Dissociation Kit, Extraction:Article Title: Protocol for minimized-bias profiling of liver and visceral adipose tissue in mice using integrated single-nucleus RNA sequencing. Article Snippet: .. Biological samples Male wild-type C57BL/6J mice, 8 weeks old at diet initiation; tissues collected after 26 weeks of western diet feeding Janvier Labs Ethics approval #47097-2024012516265646 v4 Chemicals, peptides, and recombinant proteins PBS Gibco Cat#10010023 DMEM Gibco Cat#10566016 Absolute ethanol Major supplier N/A Nuclease-free water Major supplier N/A DAPI Staining Solution MILTENYI BIOTEC Cat#130-111-570 RNase inhibitor, 40000 U/mL New England Biolabs Cat#M0314 BD Stain Buffer (FBS) BD Biosciences Cat#554656 Proteinase K, 800 U/mL New England Biolabs Cat#P8107S Vybrant® DyeCycleTM Green Stain Thermo Fisher Cat#V35004 Critical commercial assays Nuclei Extraction Buffer MILTENYI BIOTEC Cat#130-128-024 Liver Dissociation Kit, Amplification:Article Title: Protocol for minimized-bias profiling of liver and visceral adipose tissue in mice using integrated single-nucleus RNA sequencing. Article Snippet: .. Biological samples Male wild-type C57BL/6J mice, 8 weeks old at diet initiation; tissues collected after 26 weeks of western diet feeding Janvier Labs Ethics approval #47097-2024012516265646 v4 Chemicals, peptides, and recombinant proteins PBS Gibco Cat#10010023 DMEM Gibco Cat#10566016 Absolute ethanol Major supplier N/A Nuclease-free water Major supplier N/A DAPI Staining Solution MILTENYI BIOTEC Cat#130-111-570 RNase inhibitor, 40000 U/mL New England Biolabs Cat#M0314 BD Stain Buffer (FBS) BD Biosciences Cat#554656 Proteinase K, 800 U/mL New England Biolabs Cat#P8107S Vybrant® DyeCycleTM Green Stain Thermo Fisher Cat#V35004 Critical commercial assays Nuclei Extraction Buffer MILTENYI BIOTEC Cat#130-128-024 Liver Dissociation Kit, Magnetic Beads:Article Title: Protocol for minimized-bias profiling of liver and visceral adipose tissue in mice using integrated single-nucleus RNA sequencing. Article Snippet: .. Biological samples Male wild-type C57BL/6J mice, 8 weeks old at diet initiation; tissues collected after 26 weeks of western diet feeding Janvier Labs Ethics approval #47097-2024012516265646 v4 Chemicals, peptides, and recombinant proteins PBS Gibco Cat#10010023 DMEM Gibco Cat#10566016 Absolute ethanol Major supplier N/A Nuclease-free water Major supplier N/A DAPI Staining Solution MILTENYI BIOTEC Cat#130-111-570 RNase inhibitor, 40000 U/mL New England Biolabs Cat#M0314 BD Stain Buffer (FBS) BD Biosciences Cat#554656 Proteinase K, 800 U/mL New England Biolabs Cat#P8107S Vybrant® DyeCycleTM Green Stain Thermo Fisher Cat#V35004 Critical commercial assays Nuclei Extraction Buffer MILTENYI BIOTEC Cat#130-128-024 Liver Dissociation Kit, |